In which phase of mitosis do chromosomes line up at the cell's equatorial plane?

During metaphase, chromosomes align at the cell's equatorial plane, also known as the metaphase plate. This alignment is crucial because it ensures that, during the next phase of mitosis (anaphase), the sister chromatids can be pulled apart evenly towards opposite poles of the cell. The organization of chromosomes at this stage allows for accurate distribution of genetic material into the daughter cells, which is essential for maintaining the correct chromosome number in the resulting cells. The proteins that make up the spindle apparatus play a key role in facilitating this alignment and ensuring proper attachment of the chromosomes to the spindle fibers.